Shiitake Mushroom

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Ting Bao, MD, DABMA, MS

Ting Bao, MD, DABMA, MS

Jyothirmai Gubili, MS

Jyothirmai Gubili, MS

The ASCO Post’s Integrative Oncology series is intended to facilitate the availability of evidence-based information on integrative and complementary therapies commonly used by patients with cancer. In this installment, authors Ting Bao, MD, DABMA, MS, and Jyothirmai Gubili, MS, present the case study of a postmenopausal woman with early-stage estrogen receptor–positive, HER2-negative breast cancer who asks her health-care team about taking shiitake mushroom to boost her immune system.

Scientific Name: Lentinula edodes 

Common Names: Forest mushroom, lentinula, pasania fungus, black mushroom, hua gu, xian gu

Case Study

G.B. is a 61-year-old postmenopausal woman who was diagnosed with early-stage estrogen receptor–positive, HER2–negative breast cancer. She underwent lumpectomy and sentinel lymph node biopsy, finishing chemotherapy and radiation therapy 3 months ago. She is currently without evidence of disease and has been taking anastrozole, with minimal side effects. She is interested in taking shiitake mushroom to boost her immune system.


An edible mushroom native to East Asia, shiitake is cultivated worldwide for its health-promoting effects. Both the fresh and dried mushrooms are an important ingredient in East Asian cooking. Considered a medicinal mushroom, it is employed in traditional medicine and is also valued as an anticancer agent. It is generally considered to be safe to be taken by cancer survivors.

A polysaccharide derived from shiitake, lentinan, has been extensively studied and is thought to be responsible for the mushroom’s beneficial effects. It is used in an injectable form in some countries to treat cancer but has yet to be evaluated in larger studies.

Shiitake is available in Asian grocery stores and in most supermarkets. It is also available in supplemental forms, including tablets, capsules, and extracts.

The Science

Lentinan (1,3 beta-D-glucan), considered a biologic response modifier, was shown to exert anticancer effects in vitro.1 Lentin, the protein component of shiitake, demonstrated antifungal and antiproliferative properties as well as suppressed the activity of HIV-1 reverse transcriptase.2

Also, preclinical experimentation with whole shiitake extracts revealed antiproliferative, immunostimulatory,3 hepatoprotective,4 antimutagenic,5 and anticaries6 effects. Small clinical studies have indicated improvements in immune function following consumption of shiitake mushrooms in healthy young adults7; and a shiitake mycelium extract lowered the incidence of side effects associated with chemotherapy in patients with advanced gastrointestinal cancer.8 However, an orally administered shiitake extract was found to be ineffective in the treatment of prostate cancer.9


Mixed results have been reported with the use of shiitake mushrooms or extract in cancer, with reduced side effects associated with chemotherapy in those with advanced gastrointestinal cancer8 and no benefit in treating those with prostate cancer.9

Interestingly, an oral formulation of superfine dispersed lentinan has been shown to improve quality of life and survival in patients with hepatocellular carcinoma,10 gastric,11 colorectal,12 and pancreatic13 cancers in small studies.

Mechanistic studies indicate that shiitake extract–induced apoptosis involves caspase 3 and 8 pathways in hepatocellular carcinoma cells.14 Shiitake-derived polysaccharides exert antitumor effects by increasing serum interleukin-2 (IL-2) levels and tumor necrosis factor-alpha production and by inducing apoptosis,15 whereas the anticancer properties of lentinan are likely due to its ability to suppress cytochrome P450 1A enzymes, which metabolize procarcinogens to their active forms, as shown in animal studies.16 The clinical significance of these findings, however, has yet to be determined.

Adverse Reactions

Case Reports

Chronic hypersensitivity pneumonitis was observed in a patient with lung cancer following exposure to shiitake spores.17

Prolonged consumption of shiitake mushrooms resulted in dermatitis,18 photosensitivity,19 eosinophilia, and gastrointestinal upset.20

Food allergy, manifested as esophageal symptoms, was reported following consumption of shiitake mushroom.21

Small bowel obstruction, caused by ingestion of a whole shiitake mushroom, resulted in necrosis and mucosal damage in the small intestine.22

Chronic consumption of shiitake may increase eosinophil counts.20

Case Summary

Based on available evidence, we advised our patient, G.B., that it is safe to take shiitake mushroom if she wishes, but there is no evidence to indicate this will boost her immune system.

For additional information, visit the “About Herbs” website at ■

Dr. Bao is Director, Integrative Breast Oncology, Memorial Sloan Kettering Cancer Center, New York. *She is a Diplomat of the American Board of Medical Acupuncture. Ms. Gubili is Editor, Integrative Medicine, Memorial Sloan Kettering Cancer Center. 

DISCLOSURE: Dr. Bao and Ms. Gubili reported no conflicts of interest.


1. Ng ML, Yap AT: Inhibition of human colon carcinoma development by lentinan from shiitake mushrooms (Lentinus edodes). J Altern Complement Med 8:581-589, 2002.

2. Ngai PH, Ng TB: Lentin, a novel and potent antifungal protein from shitake mushroom with inhibitory effects on activity of human immunodeficiency virus-1 reverse transcriptase and proliferation of leukemia cells. Life Sci 73:3363-3374, 2003.

3. Israilides C, Kletsas D, Arapoglou D, et al: In vitro cytostatic and immunomodulatory properties of the medicinal mushroom Lentinula edodes. Phytomedicine 15:512-519, 2008.

4. Akamatsu S, Watanabe A, Tamesada M, et al: Hepatoprotective effect of extracts from Lentinus edodes mycelia on dimethylnitrosamine-induced liver injury. Biol Pharm Bull 27:1957-1960, 2004.

5. de Lima PL, Delmanto RD, Sugui MM, et al: Letinula edodes (Berk.) Pegler (Shiitake) modulates genotoxic and mutagenic effects induced by alkylating agents in vivo. Mutat Res 496:23-32, 2001.

6. Shouji N, Takada K, Fukushima K, et al: Anticaries effect of a component from shiitake (an edible mushroom). Caries Res 34:94-98, 2000.

7. Dai X, Stanilka JM, Rowe CA, et al: Consuming Lentinula edodes (Shiitake) mushrooms daily improves human immunity: A randomized dietary intervention in healthy young adults. J Am Coll Nutr 34:478-487, 2015.

8. Okuno K, Uno K: Efficacy of orally administered Lentinula edodes mycelia extract for advanced gastrointestinal cancer patients undergoing cancer chemotherapy: A pilot study. Asian Pac J Cancer Prev 12:1671-1674, 2011.

9. deVere White RW, Hackman RM, Soares SE, et al: Effects of a mushroom mycelium extract on the treatment of prostate cancer. Urology 60:640-644, 2002.

10. Isoda N, Eguchi Y, Nukaya H, et al: Clinical efficacy of superfine dispersed lentinan (beta-1,3-glucan) in patients with hepatocellular carcinoma. Hepatogastroenterology 56:437-441, 2009.

11. Oba K, Kobayashi M, Matsui T, et al: Individual patient based meta-analysis of lentinan for unresectable/recurrent gastric cancer. Anticancer Res 29:2739-2745, 2009.

12. Hazama S, Watanabe S, Ohashi M, et al: Efficacy of orally administered superfine dispersed lentinan (beta-1,3-glucan) for the treatment of advanced colorectal cancer. Anticancer Res 29:2611-2617, 2009.

13. Shimizu K, Watanabe S, Watanabe S, et al: Efficacy of oral administered superfine dispersed lentinan for advanced pancreatic cancer. Hepatogastroenterology 56:240-244, 2009.

14. Yukawa H, Ishikawa S, Kawanishi T, et al: Direct cytotoxicity of Lentinula edodes mycelia extract on human hepatocellular carcinoma cell line. Biol Pharm Bull 35:1014-1021, 2012.

15. Wang KP, Zhang QL, Liu Y, et al: Structure and inducing tumor cell apoptosis activity of polysaccharides isolated from Lentinus edodes. J Agric Food Chem 61:9849-9858, 2013.

16. Okamoto T, Kodoi R, Nonaka Y, et al: Lentinan from shiitake mushroom (Lentinus edodes) suppresses expression of cytochrome P450 1A subfamily in the mouse liver. Biofactors 21:407-409, 2004.

17. Suzuki K, Tanaka H, Sugawara H, et al: Chronic hypersensitivity pneumonitis induced by Shiitake mushroom spores associated with lung cancer. Intern Med 40:1132-1135, 2001.

18. Garg S, Cockayne SE: Shiitake dermatitis diagnosed after 16 years! Arch Dermatol 144:1241-1242, 2008.

19. Hanada K, Hashimoto I: Flagellate mushroom (Shiitake) dermatitis and photosensitivity. Dermatology 197:255-257, 1998.

20. Levy AM, Kita H, Phillips SF, et al: Eosinophilia and gastrointestinal symptoms after ingestion of shiitake mushrooms. J Allergy Clin Immunol 101:613-620, 1998.

21. Goikoetxea MJ, Fernández-Benítez M, Sanz ML: Food allergy to Shiitake (Lentinus edodes) manifested as oesophageal symptoms in a patient with probable eosinophilic oesophagitis. Allergol Immunopathol (Madr) 37:333-334, 2009.

22. Kusumoto M, Koganemaru M, Nakayama G, et al: Dietary small bowel obstruction. BMJ Case Rep Jan 25;2013, 2013.