Novel Drug Combinations Targeting Pathways Triggered by KRAS May Benefit Patients With Pancreatic Cancer


Key Points

  • Dinaciclib combined with investigational drugs blocking two of the major signaling pathways triggered by KRAS inhibited tumor growth and reduced metastasis in mouse models of pancreatic cancer, compared with either drug alone.
  • Treatment with dinaciclib and MK2206, which blocks the PI3K/AKT pathway, led to complete responses in 3 of the 14 mice.
  • A phase I clinical trial evaluating dinaciclib plus MK2206 in patients with pancreatic cancer has been initiated based on these preclinical findings.

Two drug combinations that simultaneously block two major signaling pathways downstream of the protein KRAS, which is aberrantly active in most pancreatic cancers, may provide a new treatment option for patients with this disease, according to preclinical results presented at the AACR-NCI-EORTC International Conference on Molecular Targets and Cancer Therapeutics, held October 19 to 23 in Boston (Abstract B263).

Pancreatic cancer is one of the deadliest types of cancer, with a 5-year survival rate of just 6%. Because these mutations in the KRAS gene drive many of the cancerous characteristics of pancreatic cancer cells, the KRAS protein is a prime therapeutic target. However, efforts to develop clinically useful drugs that block KRAS activity have been unsuccessful.

Targeting Pathways

“KRAS has been a daunting therapeutic target,” said Barry Nelkin, PhD, Professor of Oncology at the Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins in Baltimore. “By combining drugs that simultaneously block two of the major signaling pathways triggered by KRAS, we have found a way to indirectly target this challenging protein.

“Our preclinical results were so positive that we have initiated a phase I clinical trial to evaluate one of the drug combinations, dinaciclib plus MK2206, in patients with pancreatic cancer.”

In prior studies, Dr. Nelkin and colleagues found that an investigational drug called dinaciclib had antitumor effects in mouse models of pancreatic cancer.

“Dinaciclib blocks the activity of a protein called CDK5, which is a key part of one of the signaling pathways that KRAS uses to exert its cancer-driving effects, the Ral pathway,” explained Dr. Nelkin. “We wanted to investigate whether combining dinaciclib with a second drug that blocks one of the other signaling pathways triggered by KRAS would have even greater antitumor effects.”

To conduct the study, Dr. Nelkin and colleagues used models of pancreatic cancer that closely resembled the human disease: They placed tiny pieces of human pancreatic tumors in the pancreata of mice and let them grow to about the size of a pea before beginning treatment.

Combination Treatment Reduces Tumor Growth

The combination of dinaciclib and MK2206, which blocks the PI3K/AKT pathway triggered by KRAS, substantially inhibited tumor growth and reduced metastasis, compared with either drug alone. Further, when compared with no treatment, this drug combination reduced tumor growth by 90%, and in 3 of the 14 mice, no human pancreatic tumor tissue could be detected at the end of the experiment, indicating that there had been a complete response to the treatment.

The second drug combination tested—dinaciclib and SCH772984, which blocks the RAF/MEK/ERK pathway triggered by KRAS—also substantially inhibited tumor growth and reduced the number of metastases. It did not, however, lead to any complete responses.

The researchers are planning to investigate whether the drug combinations they used in this study can be further combined with current treatments for pancreatic cancer and if they can identify markers that might predict whether a given pancreatic tumor will respond to the drug combinations.

This study was funded by the National Cancer Institute.

Dr. Nelkin reported no potential conflicts of interest.

The content in this post has not been reviewed by the American Society of Clinical Oncology, Inc. (ASCO®) and does not necessarily reflect the ideas and opinions of ASCO®.