Vaccine Stirs Immune Activity Against Advanced Chronic Lymphocytic Leukemia
Patients with advanced chronic lymphocytic leukemia (CLL) often receive allogeneic transplants that effectively “reboot” their own immune defenses, which then attack and potentially cure the hard-to-treat disease. However, there is a high rate of relapse in these patients, and the transplanted immune cells may also harm normal tissues, causing graft-vs-host disease.
Now, scientists at Dana-Farber Cancer Institute report in the Journal of Clinical Investigation that they observed a strong and selective immune response in some patients who received, shortly after the transplant, several doses of a “personalized” tumor vaccine composed of their own inactivated leukemia cells combined with an immune stimulant, granulocyte-macrophage colony-stimulating factor (GM-CSF, Leukine). Thus the vaccine boosted the power of the transplanted immune system’s ability to attack the cancer—known as the graft-vs-leukemia effect.
“Our studies suggest that autologous tumor cell vaccination is an effective strategy to advance long-term leukemia control” following transplants from donors, said senior author Catherine Wu, MD. “Although this was a phase I study and not powered to look at questions of clinical efficacy, we did see promising clinical activity.”
Promising Treatment Option
There are few treatment options for advanced CLL. Standard transplants, which involve powerful doses of pretransplant chemotherapy to wipe out as much of the leukemia as possible, have proven too toxic for older patients and those with coexisting diseases. Over the past decade, researchers have developed reduced-intensity conditioning regimens, using lower chemotherapy doses that are more tolerable but which rely entirely on the activity of the transplanted immune cells to battle the leukemia. Usually this is insufficient to keep the cancer at bay long-term, and the disease progresses.
Furthermore, research has shown that the identifying antigens on the surface of CLL cells in individual patients may differ—that is, they have “personal tumor antigens,” the scientists said. “Based on these principles, vaccination with autologous irradiated leukemia cells is an attractive approach to expand leukemia-reactive T cells, since this vaccine formulation reliably includes personal tumor antigens,” they wrote.
To make the vaccine, the researchers mixed the patients’ irradiated leukemia cells with cells that produce GM-CSF and then injected them back into the patient. The combination stirs up a strong response by immune T cells, and the distinctive antigens on the injected leukemia cells direct the T cells to attack similar leukemia cells wherever they are present in the body.
Phase I Trial
In the phase I trial, the vaccine was administered between 30 and 100 days after the transplant, with some patients receiving as many as six vaccine doses. The study enrolled 22 patients with advanced, aggressive CLL. Thirteen of the patients had evidence of the leukemia in their bone marrow at the time of transplant.
Four patients did not receive the vaccine because they developed graft-vs-host disease following the transplant. The remaining 18 received at least one vaccine dose; 7 patients stopped receiving the vaccine after they developed graft-vs-host disease.
When examined 6 months post-transplant, the majority of patients showed evidence of clinical response: 10 had complete remissions, and 6 had partial remissions. After a median follow-up of 2.9 years, 13 patients (72%) had remained in continuous complete remission; 1 patient had stable disease, 3 patients developed progressive disease, and 2 of those patients died.
The results support the safety and biologic activity of whole tumor-cell vaccination in hematologic malignancies, said the authors, and that giving the vaccine shortly after transplant may have been critical in its effectiveness. In addition, they said a key to the vaccine’s potency was the development of GM-CSF–secreting “bystander” cells that can be used against lymphoid malignancies, which was not possible previously.
However, the authors noted that further randomized studies in larger patient groups will be necessary to determine if this strategy “will translate into a true clinical benefit for patients with advanced CLL.”
The research was supported by National Cancer Institute (5R21CA115043-2); National Heart, Lung, and Blood Institute (5R01HL103532-03); the Claudia Adams Barr Program in Cancer Research; the Leukemia and Lymphoma Society Translational Research Program; and an Early Career Physician-Scientist Award of the Howard Hughes Medical Institute. Dr. Wu is a Damon Runyon Clinical Investigator supported in part by the Damon Runyon Cancer Research Foundation.
The content in this post has not been reviewed by the American Society of Clinical Oncology, Inc. (ASCO®) and does not necessarily reflect the ideas and opinions of ASCO®.
